Bacterial CellCultures in a Lab-on-a-Disc: a Easy and Versatile Device for Quantification of Antibiotic

Bacterial CellCultures in a Lab-on-a-Disc: a Easy and Versatile Device for Quantification of Antibiotic

Nieuwste ontwikkelingen in kunstmatige  biologie – ingeschakelde en pure  optische biosensing van zware metalen in hele cellen cell

Er zijn veel wetenschappelijke werken gedrukt op biosensing van zwaar staal met hele cellen, voornamelijk gebaseerd op optische transductie. De vooruitgang binnen de software van biotechnologische instrumenten heeft niet alleen de gevoeligheid, selectiviteit en detectie voor biosensoren herhaaldelijk verbeterd, maar heeft tegelijkertijd nieuwe uitdagingen en beperkingen voor aanvullende verbeteringen aan het licht gebracht.

Deze evaluatie benadrukt de gekozen kenmerken van biosensing van hele cellen van zware metalen met behulp van optische transducers. Nu hebben we ons gecentreerd op de vooruitgang in genetische modulatie in regulerende en reportermodules van recombinante plasmiden die de verbetering van de efficiëntie van biosensoren mogelijk hebben gemaakt.

Tegelijkertijd is er een poging gedaan om de huidige nieuwere platforms te gebruiken die gelijkwaardig zijn aan microfluïdica, die veelbelovende resultaten hebben opgeleverd en een geheel nieuwe draai kunnen geven aan het optische biosensing-gebied.

Bacteriële celculturen in een Lab-on-a-Disc: een eenvoudig en veelzijdig systeem voor kwantificering van de werkzaamheid van antibioticabehandeling

Pathogene bacteriële biofilms zullen waarschijnlijk levensbedreigend zijn, de kwaliteit van leven van de getroffen persoon enorm verminderen en een aanzienlijke belasting vormen voor het gezondheidszorgsysteem. De huidige methoden voor evaluatie van antibacteriële therapieën in klinieken en in vitro-strategieën die worden gebruikt bij de ontwikkeling en screening van geneesmiddelen, vergemakkelijken elk de vorming van biofilms niet of zijn omslachtig om uit te voeren, vereisen enorme reagensvolumes en zijn kostbaar, waardoor hun bruikbaarheid wordt beperkt.

Om met deze factoren om te gaan, presenteert dit werk de gelegenheid van een stevig in vitro celaangepast platform dat geschikt is voor confocale microscopie.

Het platform, gevormd als een compact disc, maakt gebruik van bacteriën op lange termijn mogelijk zonder externe pompen en slangen en zal waarschijnlijk vele dagen worden gebruikt zonder verdere vloeistof. Pseudomonas aeruginosa-biofilm wordt bijvoorbeeld gekweekt uit afzonderlijke cellen en er is bevestigd dat:

1) het platform levert reproduceerbare en betrouwbare resultaten; 2) de voortgang is afhankelijk van de bewegingskosten en de samenstelling van het voortgangsmedium; en drie) de werkzaamheid van antibiotische behandeling zal afhangen van de gevormde biofilm. Dit platform maakt de voortgang, kwantificering en behandeling van biofilm mogelijk zoals in een standaard bewegingsopstelling, terwijl de apparatuurbarrière van lab-on-chip-strategieën wordt verlaagd . Het biedt een gebruiksvriendelijk, redelijk geprijsd risico voor eindklanten die werken met celkweek in relatie tot bijvoorbeeld diagnostiek en drugsscreening.

microdish
microdish

Bead Sample Pack - 5M Set

BSP-5Y 1pack
EUR 289.2
Description: Bead sample pack for Bullet Blender 5mL Storm models. Includes 10mL of: ZROB015, ZROB05, ZROB20, SSB02, SSB14B, SSB32, SSB60, SSB110 and SSUFO35.

Bead Sample Pack - 50mL Set

BSP-50B2 1pack
EUR 484.8
Description: Bead sample pack for Bullet Blender 50mL tube models. Includes 20mL of: ZROB05, ZROB20, SSB02, SSB14B, SSB48, SSB60, SSUFO35 and SSUFO56.

Bead Sample Pack - Full Set

BSP-ALL2 1pack
EUR 460.8
Description: Bead sample pack inlcudes sampling of all 20 bead types in 10mL tubes.

Bead Sample Pack - Organ Tissues

BSP-OT3 1pack
EUR 282
Description: Bead sample pack for homogenization of organ tissues. Includes 10mL of: ZROB05, ZROB10, ZROB20, SSB14B, SSB32 and SSUFO35.

Bead Sample Pack - Microcentrifuge Set

BSP-MC2 1pack
EUR 278.4
Description: Bead sample pack for Bullet Blender 1.5mL tube models. Includes 10mL of: ZROB015, ZROB05, ZROB10, ZROB20, SSB02, SSB14B, SSB32 and SSUFO35.

Water, 0.1um Filtered, cell cultures tested

W0900-050 500 ml
EUR 81.6

Water, 0.1um Filtered, cell cultures tested

W0900-310 10x500 ml
EUR 202.8

Water, 0.1um Filtered, cell cultures tested

W0900-350 50x500 ml
EUR 627.6

Red Blood Cell Sample Pack

88R-1050 6 x 2 ml
EUR 483.6
Description: Horse, Mouse, Rabbit, Sheep, Bovine and Chicken glutaraldehyde stabilized blood cell samples

CULTURE VESSEL, C1770 SAMPLE

C2099 10EA
EUR 10.56

All Sample Diluents Sample Pack

KF17355 3X100 ml
EUR 326.4

PTcon SAMPLE PACK

C2125 5EA
EUR 32.34

Growth Supplement for Anaerobic Cultures

FD805-100G 1 unit
EUR 16.61
Description: Growth Supplement for Anaerobic Cultures

GELLING AGENT SAMPLE PACK

G3500 1EA
EUR 17.71

3 Blocking Buffer Sample Pack

KF17340 3 x 100 ml
EUR 246

All Assay Diluents Sample Pack

KF17351 4X100 ml
EUR 326.4

TMB Peroxidase Substrate SAMPLE PACK

42R-TB101 3 x 100 ml
EUR 393
Description: 100mL of 3 different TMB Peroxidase Substrates ready to use in immunoassays: Ultra sensitivite, Highly Sensitive, and Slow Acting.

GC/HPLC Demo Sample Pack - EACH

TNDPSSSV EACH
EUR 5.4

GC Headspace Demo Sample Pack - EACH

TNDPSSHS EACH
EUR 5.4

96 Well Cell Culture Plate, White, Flat bottom, TC, Sterile, 1/pack, 100/cs

701301 100 pcs/cs
EUR 103.73
Description: 96 Well Cell Culture Plate, White, Flat bottom, TC, Sterile, 1/pack, 100/cs

Cell Culture Flask,3 Layer, Vent Cap, TC, Sterile,Bag Package, 1 pc/pack, 12 pak

731302 12 pcs/cs
EUR 65.89
Description: Cell Culture Flask,3 Layer, Vent Cap, TC, Sterile,Bag Package, 1 pc/pack, 12 pakcs/case, 12 pcs/case

S-adenosylmethionine ELISA kit for cell culture supernatant or other samples

IK00204 96 tests
EUR 699
Description: S-adenosylmethionine ELISA kit for cell culture supernatant or other samples

Cell Culture Flask,3 Layer, Plug Seal Cap, TC, Sterile,Bag Package, 1 pc/pack, 1

731301 12 pcs/cs
EUR 64.9
Description: Cell Culture Flask,3 Layer, Plug Seal Cap, TC, Sterile,Bag Package, 1 pc/pack, 12 pakcs/case, 12 pcs/case

S-adenosylhomocysteine ELISA kit for cell culture supernatant or other samples

IK00304 96 tests
EUR 1049
Description: S-adenosylhomocysteine ELISA kit for cell culture supernatant or other samples

Supreme Plant Tissue Culture Grade Agar

PCTA100 500g
EUR 144
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

Supreme Plant Tissue Culture Grade Agar

PCTA500 1000g
EUR 180
Description: A polysaccharide complex that is produced from the red alga Rhodophyceae. The extraction of agarocytes is obtained by bleaching and hot water. Supreme Plant Tissue Culture Grade Agar offers greater clarity of plant culture media.

CORNING® BIOCOAT™ POLY-D-LYSINE/LAMININ 8 WELL CULTURESLIDE, 4/PACK, 12/CASE

354688 3/pk
EUR 543.6
Description: Advanced Cells - DL; Slips/Slides

96 Well Cell Culture Plate, White, Flat bottom, Non-Treated, Sterile, 1/pack, 10

701311 100 pcs/cs
EUR 101.75
Description: 96 Well Cell Culture Plate, White, Flat bottom, Non-Treated, Sterile, 1/pack, 100/cs

Trueprep® AUTO MTB Sample Pre-treatment Pack

60204AS05 5T
EUR 5

Trueprep® AUTO MTB Sample Pre-treatment Pack

60204AS20 20T
EUR 20

Trueprep® AUTO MTB Sample Pre-treatment Pack

60205AS50 50T
EUR 50

Sample Diluent Optimization Pack: 100mL SD1, SD2, SD3, PFSD

959 pack
EUR 187

Trueprep® AUTO Universal Sample Pre-treatment Pack

60205AB05 5T
EUR 5

Trueprep® AUTO Universal Sample Pre-treatment Pack

60205AB20 20T
EUR 20

Trueprep® AUTO Universal Sample Pre-treatment Pack

60205AB50 50T
EUR 50

Multifuge X4 Pro cell culture package - EACH

75016067 EACH
EUR 18158.85

Multifuge X4R Pro cell culture package - EACH

75016066 EACH
EUR 24460.65

SL4R Plus Centrifuge Cell Culture TX1000 Package - EACH

75016075 EACH
EUR 20652.3

Protein A Tell-Tale Gold® Ribbon Sample Pack, 3 x (1 cm x 30 cm)

TTGA-SP3-B090 3 x (1 cm x 30 cm)
EUR 208.7

Protein G Tell-Tale Gold® Ribbon Sample Pack, 3 x (1 cm x 30 cm)

TTGG-SP3-B090 3 x (1 cm x 30 cm)
EUR 208.7

Corning 100mm TC-Treated Culture Dishes in 6-pack Carriers - PK480

430293 PK480
EUR 571.05

Protein A/G Tell-Tale Gold® Ribbon Sample Pack, 3 x (1 cm x 30 cm)

TTGAG-SP3-B090 3 x (1 cm x 30 cm)
EUR 208.7

CORNING® BIOCOAT™ POLY-D-LYSINE 60MM CULTURE DISHES, 20/PACK, 100/CASE, NONSTERILE

356468 20/pk
EUR 469.2
Description: Advanced Cells - DL; Dishes BCT

Gryphon Ecotropic Packaging Cell Line for Retrovirus (1 x 106 cells, Culture)

ABP-RVC-10002C 1 flask Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

CORNING® BIOCOAT™ COLLAGEN I 35MM TC-TREATED CULTURE DISHES, 100/PACK, 100/CASE, NONSTERILE

356456 20/pk
EUR 469.2
Description: Advanced Cells - DL; Dishes BCT

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 770

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 770

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 770

Gryphon Amphotropic Packaging Cell Line for Retrovirus (1 x 106 cells, Culture)

ABP-RVC-10001C 1 flask Ask for price
Description: PRODUCT LINE DISCONTINUED. Please contact us.

Cell Culture Grade Water, Cell Culture Tested, Sterile

CCM1081-500 500 mL
EUR 73.49

Cell Culture Grade Water, Cell Culture Tested, Sterile

CCM2081-1000 1000 mL
EUR 82.64

MDR Sampler Pack antibody

STJ16100969 1 pack
EUR 799.2

Magnetic COOH Sampler Pack

BLI21940-1 5ml
EUR 395

CORNING® BIOCOAT™ POLY-D-LYSINE 35MM TC-TREATED CULTURE DISHES, 20/PACK, 100/CASE, NONSTERILE

356467 20/pk
EUR 468
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ POLY-L-LYSINE 60MM TC-TREATED CULTURE DISHES, 20/PACK, 100/CASE, NONSTERILE

356517 20/pk
EUR 481.2
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ POLY-L-LYSINE 35MM TC-TREATED CULTURE DISHES, 20/PACK, 100/CASE, NONSTERILE

356518 20/pk
EUR 481.2
Description: Advanced Cells - DL; Dishes BCT

Sample Cell 10ml Matched - PK2

2495402 PK2
EUR 460.35

CORNING® BIOCOAT™ LAMININ 35MM TC-TREATED CULTURE DISHES, 5/PACK, 20/CASE, NONSTERILE

354458 20/pk
EUR 267.6
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ GELATIN 100MM TC-TREATED CULTURE DISHES, 10/PACK, 40/CASE, NONSTERILE

356653 10/pk
EUR 262.8
Description: Advanced Cells - DL; Dishes BCT

Magnetic Streptavidin Sampler Pack

BLI21950-1 1ml
EUR 342

Water for Cell Culture

TBS5050 500ml
EUR 10

100 mm Cell Culture Dish

P0300 300/case
EUR 250

Europium Chelate COOH Sampler Pack

BLI21960-1 1ml
EUR 520

CORNING® BIOCOAT™ MATRIGEL® MATRIX THIN LAYER 100MM CULTURE DISHES, 10/PACK, 10/CASE, NONSTERILE

354600 5/pk
EUR 291.6
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ MATRIGEL® MATRIX THIN LAYER 60MM CULTURE DISHES, 5/PACK, 20/CASE, NONSTERILE

354601 5/pk
EUR 330
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ MATRIGEL® MATRIX THIN LAYER 35MM CULTURE DISHES, 20/PACK, 20/CASE, NONSTERILE

354602 10/pk
EUR 304.8
Description: Advanced Cells - DL; Dishes BCT

CORNING® BIOCOAT™ POLY-D-LYSINE 100MM TC-TREATED CULTURE DISHES, 10/PACK, 40/CASE, NONSTERILE

356469 10/pk
EUR 262.8
Description: Advanced Cells - DL; Dishes BCT

T175 Cell Culture Flask

P0220 40/case
EUR 215

T225 Cell Culture Flask

P0230 25/case
EUR 185

Cell Culture Grade Water

25-511 6 x 500 mL/Unit
EUR 52.91
Description: Cell Culture Tested, Sterile

Cell Culture Grade Water

25-511B 6 x 1000 mL
EUR 85.86
Description: Cell Culture Tested, Sterile

Water, Cell Culture Grade

CA018-600 6x1000ml
EUR 148.8

Hepes Cell Culture Grade

AK3268-0250 250g Ask for price

Hepes Cell Culture Grade

AK3268-0500 500g Ask for price

Hepes Cell Culture Grade

AK3268-1000 1kg Ask for price

CELL CULTURE GRADE WATER

IB42010 1L
EUR 41.13
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42011 6x1L
EUR 239.38
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42012 12x1L
EUR 469.02
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42020 2L
EUR 59.67
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42021 6x2L
EUR 342.87
Description: DBL DIST. - 0.1 MICRON STERILE

CELL CULTURE GRADE WATER

IB42030 10L
EUR 194.72
Description: JUG W/HANDL 0.1 MICRON STERILE

Sample Cell 1 Inch Round PS - PK2

WAT1120 PK2
EUR 63.45

ROCKER CELL CULTURE BAG, 2L

91-200-80 1/pk
EUR 450
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 20L

91-200-78 1/pk
EUR 493.2
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 10L

91-200-79 1/pk
EUR 446.4
Description: Flexible Containers; Bioprocess Bags and Parts

ROCKER CELL CULTURE BAG, 50L

91-200-81 1/pk
EUR 570
Description: Flexible Containers; Bioprocess Bags and Parts

Mouse ES Cell Culture Media

M16101 500 mL
EUR 1336.8

CRFK CELL TUBE CULTURE

63-0600 each Ask for price

Sample Cell 1 Inch Round Glass - PK6

WAT1118 PK6
EUR 156.6

6-well Cell Culture Plates

abx090688-50plates 50 plates
EUR 571.2

6-well Cell Culture Plates

abx090688-6plates 6 plates
EUR 142.8

6-Well Cell Culture Plates

25-105 100 Plates/Unit
EUR 119.75
Description: Flat Bottom Wells, TC Treated

HEPES. cell culture tested - kg

PM-B2093/1000 kg
EUR 146.69

Human MEP Cell Culture Medium (human megakaryocyte–erythroid progenitor cell culture medium)

P770 8 rxn
EUR 499

3D Cell Culture Matrix BME Kit

K518-100 each
EUR 744

12-Well Cell Culture Plates

25-106 100 Plates/Unit
EUR 123.27
Description: Flat Bottom Wells, TC Treated

24-Well Cell Culture Plates

25-107 100 Plates/Unit
EUR 125.99
Description: Flat Bottom Wells, TC Treated

48-Well Cell Culture Plates

25-108 100 Plates/Unit
EUR 138.07
Description: Flat Bottom Wells, TC Treated

96-Well Cell Culture Plates

25-109 100 plates
EUR 117.72
Description: Flat Bottom Wells, TC Treated

96-Well Cell Culture Plates

25-221 100 plates
EUR 139.75
Description: U-Bottom Wells, TC Treated

Cell Culture Inserts, 6-Well

25-281 6 Inserts/Plate, 24/Unit
EUR 71.41
Description: 1µm PC Membrane, Sterilized

Cell Culture Inserts, 6-Well

25-282 6 Inserts/Plate, 24/Unit
EUR 71.41
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 6-Well

25-283 6 Inserts/Plate, 24/Unit
EUR 71.41
Description: 8µm PC Membrane, Sterilized

Fish Serum (cell culture grade)

GWB-AE89C9 10 ml Ask for price

HEPES. cell culture tested - 100g

PM-B2093/100 100g
EUR 23.21

HEPES. cell culture tested - 250g

PM-B2093/250 250g
EUR 44

HEPES. cell culture tested - 500g

PM-B2093/500 500g
EUR 80.08

Cell Culture Inserts, 12-Well

25-285 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 12-Well

25-286 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 8µm PC Membrane, Sterilized

Cell Culture Inserts, 24-Well

25-287 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 1µm PC Membrane, Sterilized

Cell Culture Inserts, 24-Well

25-288 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 3µm PC Membrane, Sterilized

Cell Culture Inserts, 24-Well

25-289 12 Inserts/Plate, 48/Unit
EUR 112.46
Description: 8µm PC Membrane, Sterilized

ITS Cell Culture Supplement(100×)

ITSS-10201 10mL
EUR 59

Human CD34+ Cell Culture Medium

P771 8 rxn
EUR 299

Long-term Cell Tracer 580 (Green) sample

P710GS 0.1 ml
EUR 199

DMSO Bio-Max, Cell Culture Grade

40470005-1 50 mL
EUR 94.72

DMSO Bio-Max, Cell Culture Grade

40470005-2 100 mL
EUR 158.19

DMSO Bio-Max, Cell Culture Grade

40470005-3 25 mL
EUR 53.53

DMSO Bio-Max, Cell Culture Grade

40470005-4 250 mL
EUR 271.78

resDetect™ resDNA Sample Preparation Kit (Magnetic Beads)

OPA-R005 50preps
EUR 310.3
Description: DNA extraction is a critical step in many molecular biology techniques, such as PCR, sequencing, and cloning, as it is necessary to obtain pure and intact DNA that can be used in downstream applications. DNA extraction kits can simplify and standardize the DNA extraction process, allowing researchers to obtain high-quality DNA from a wide range of samples with reproducible results.

Long-term Cell Tracer 580 (Yellow) sample

P710YS 0.1 ml
EUR 199

EZ-LINE Cell culture Dishes Tissue culture treated, 60X15mm, 500 per case

SP31171 1CS, 500UNIT
EUR 230.89

EZ-LINE Cell culture Dishes Tissue culture treated, 90x20mm, 200 per case

SP31212 1CS, 200UNIT
EUR 162.61

1L Water Cell Culture Grade WFI - PK6

25-055-CM PK6
EUR 95.85

3D Cell Culture Gel, hard (Col-Tgel)

P720H-10 10 mL
EUR 389

3D Cell Culture Gel, hard (Col-Tgel)

P720H-2 2 mL
EUR 189

3D Cell Culture Gel, soft (Col-Tgel)

P720S-10 10 mL
EUR 389

3D Cell Culture Gel, soft (Col-Tgel)

P720S-2 2 mL
EUR 189

500mL Water Cell Culture Grade - PK6

25-055-CV PK6
EUR 66.15

Gibco CHO Cell Culture Medium - EACH

220253 EACH
EUR 64.48

Ethanolamine, Cell Culture tested

CA071-010 100ml
EUR 116.4

3D Cell Culture Gel, medium (Col-Tgel)

P720M-10 10 mL
EUR 389

3D Cell Culture Gel, medium (Col-Tgel)

P720M-2 2 mL
EUR 189

3D Cell Culture Non-Enzymatic Cell Harvesting Kit

K982-100 each
EUR 326.4

Cell Culture Water Pyrogen free - 100ml

LM-T1707/100 100ml
EUR 2.59

Cell Culture Water Pyrogen free - 1000ml

LM-T1707/1000 1000ml
EUR 5.45

Cell Culture Water Pyrogen free - 500ml

LM-T1707/500 500ml
EUR 3.19

Cancer Antigen 50 (CA 50) GI cell culture

GWB-3F2336 50000 Unit Ask for price

UltraPure Water, Cell Culture Tested

W9200-045 450ml
EUR 96

3D Cell Culture HTS Cell Viability Complete Assay Kit

K948-100 each
EUR 411.6

BD Biocoat 10cm cell culture dish - PK10

354469 PK10
EUR 126.9

Cell Culture Supernatant Diluent Kit

DKC100 96 Tests
EUR 50

CardioDISC (Scaffold for cell culture)

C-01-01-EX 12 sheets Ask for price
Description: The CardioDISC (Scaffold for cell culture) is available in Europe and for worldwide shipping via Gentaur.

Human B Cell Culture and Expansion Kit

BC1001-1 1 package
EUR 5200

3D Cell Culture Matrix Duo-Matrix Kit

K519-100 each
EUR 718.8

Multi-Layer Cell Culture Bioreactor

P0700 1
EUR 415

MDR Sampler Pack (JSB-1,LRP-56,MRPm6,MRPr1)

MC-898 JSB-1, LRP-56, MRPm6, MRPr1 Ask for price

Pluronic® F-127 *Cell culture tested *

20050 10 g
EUR 88
Description: Pluronic® F-127 is a nonionic surfactant that is 100% active and relatively non-toxic to cells, and frequently used with dye AM esters to improve their water solubility.

Pluronic® F-127 *Cell culture tested *

20050-10g 10 g
EUR 86
Description: Pluronic® F-127 is a nonionic surfactant that is 100% active and relatively non-toxic to cells, and frequently used with dye AM esters to improve their water solubility.

40X9mm Cell Culture Plastic Dishes 20/PK

DS001-40 1PK, 20UNIT
EUR 64.18

Gelatin Solution , 0.1%, Cell culture tested

CA087-100 1L
EUR 116.4

Pluronic F-68, 10% Sol, Cell Culture tested

CA072-010 100ml
EUR 105.6

60X12mm Cell Culture Plastic Dishes 20/PK

DS001-60 1PK, 20UNIT
EUR 64.7

90X15mm Cell Culture Plastic Dishes 10/PK

DS001-90 1PK, 10UNIT
EUR 65.02

Mouse Epithelial Cell culture Medium Kit

ABM-TM4285 500 ml Basal medium with the growth factor supplement Ask for price
Description: Epithelial Cell Medium is a complete medium designed for the culture of epithelial cells. It was tested and optimized with epithelial cell growth and proliferation in vitro. It is formulated for use with 5% CO2 and 95% air in a humidified incubator.The medium consists of 500 ml of basal medium (containing essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals), supplemented with epithelial cell growth supplement, antibiotics, and fetal bovine serum.Epithelial Cell Medium Supplement Kit includes:- 0.5 ML RI-Transferrin-Selenium (ITS)- 0.5 ML EGF- 5.0 ML L-Glutamine- 5.0 ML Antibiotic-Antimycotic Solution- 10.0 ML FBS*Before use, add Supplement Kit into 500ml basal medium.

Probenecid *Cell culture tested* *CAS 57-66-9*

20060-10x72mg 10x72 mg
EUR 86
Description: Probenecid is an inhibitor of organic-anion transporters located in cell membranes.

Ampicillin sodium salt, cell culture grade

GA0283-100 100
EUR 189.8

Ampicillin sodium salt, cell culture grade

GA0283-100G 100 g
EUR 265.2

Ampicillin sodium salt, cell culture grade

GA0283-25 25
EUR 71.1

Ampicillin sodium salt, cell culture grade

GA0283-25G 25 g
EUR 122.4

Ampicillin sodium salt, cell culture grade

GA0283-5 5
EUR 23.8

Ampicillin sodium salt, cell culture grade

GA0283-5G 5 g
EUR 64.8

Sodium Bicarbonate. cell culture tested - kg

PM-S2011/1 kg
EUR 10.4

Ultrafiltration Water, cell culture level

PB180518-500mL 500 mL
EUR 15
Description: Supplements & Reagents

Raf-1 RBD Agarose Beads

STA-410 400 ?g
EUR 485

PAK1 PBD Agarose Beads

STA-411 400 ?g
EUR 485

GGA3 PBD Agarose Beads

STA-419 400 µg
EUR 485

Water, GlenCell™, suitable for cell culture

GK3940-1 1
EUR 55.4

Helder microkristallijn cellulose/polyvinylalcoholpapier als nieuw platform voor 3D CellCulture

Meerlagig en gestapeld cellulosepapier is naar voren gekomen als een veelbelovend platform voor het construeren van driedimensionale (3D) celgebruiken die toe te schrijven zijn aan de lage waarde, goede biocompatibiliteit en extreme porositeit. Desalniettemin maakt de slechte zachte transmissie het moeilijk om celgedrag (bijv. Vooruitgang en proliferatie) op het op papier gebaseerde platform onmiddellijk en duidelijk te volgen met behulp van een optische microscoop.

Op dit werk ontwikkelden we een transparant microkristallijn cellulose/polyvinylalcohol (MCC/PVA) papier met onregelmatige poriën door oplossing en regeneratie van microkristallijne nanocellulose (MCC), toevoeging van porogeenreagens (NaCl) en vervolgens dompelen in PVA-keuzes. Het heldere MCC-papier vertoont extreme porositeit (tot 90%), instelbare poriemeting (tussen 23 μm en 46 m) en grote dikte (van 315 m tot 436 m) en extreem zachte transmissie onder water (> 95%).

  • Door verdere modificatie van helder MCC-papier met PVA, vertoont het verkregen heldere MCC/PVA-papier verbeterde mechanische eigenschappen (droge en vochtige sterktes), goede hydrofiliciteit (met een contacthoek van 70,8°) en verbeterde biocompatibiliteit (cellevensvatbaarheid maar liefst 90 %). Door een groot aantal lagen van het heldere MCC/PVA-papier te stapelen en te ontstapelen, is het gebruikt voor elk aangepast 2D- en 3D-celplatform.

 

  • Het heldere MCC/PVA-papier onder water maakt elke directe bevestiging van celmorfologie door optische microscoop door middel van blote ogen en fluorescentiemicroscoop na kleuring mogelijk. We stellen ons voor dat het ontwikkelde heldere MCC/PVA-papier een goed potentieel biedt voor toekomstige functies in een flink aantal bioanalytische en biomedische gebieden, verwant aan het screenen van geneesmiddelen, weefseltechnologie en orgaan-op-chips.

Natuurgelijke verbindingen en zuivere zuren verbeteren en stoppen de schade veroorzaakt door een inflammatoire keerzijde in Caco-2 CellCulture

Bioactieve verbindingen, verwant aan zuivere zuren (OA) en natuur-identieke verbindingen (NIC), kunnen een taak uitoefenen binnen de veiligheid van de efficiëntie van het darmslijmvlies, die toe te schrijven is aan hun natuurlijke eigenschappen.

Het doel van dit onderzoek was om inzicht te krijgen in de prestaties van twee OA (citroenzuur en sorbinezuur) en een paar NIC (thymol en vanilline), alleen of gemengd in een mengsel (OA + NIC), op de efficiëntie van de darmbarrière, elk door homeostatisch scenario of allemaal via een inflammatoir nadeel uitgevoerd met pro-inflammatoire cytokines en lipopolysacchariden (LPS). Het onderzoek naar werd uitgevoerd op de menselijke epitheelcellijn Caco-2, een bekend model van de darmepitheelbarrière.

De resultaten bevestigden hoe OA en NIC alleen de transepitheliale elektrische weerstand (TEER) en mRNA-bereiken van tight junction (TJ) -elementen kunnen verbeteren, niettemin bevestigden OA + NIC een sterkere werkzaamheid in vergelijking met de afzonderlijke moleculen .

Wanneer er een inflammatoir nadeel optrad, was OA + NIC-combinatie in staat om de schade toe te schrijven aan de pro-inflammatoire stimulus te verbeteren en te stoppen, de daling in TEER te verminderen of te stoppen en de TJ-mRNA-expressie te verbeteren. De kennis helpt de prestaties van OA + NIC bij het moduleren van de efficiëntie van de darmbarrière en het verminderen van de tegenovergestelde uitkomsten van irritatie in darmepitheelcellen, waardoor de efficiëntie van de darmbarrière wordt ondersteund.

Prigrow VI Medium

TM016 500ml
EUR 145

Prigrow IX Medium

TM019 500ml
EUR 145

Prigrow XV Medium

TM027 500 ml
EUR 145

Prigrow CI Medium

TM101 500ml
EUR 145

Prigrow X.1 Medium

TM010 1 Kit
EUR 385

Prigrow XII Medium

TM012 500ml
EUR 125

Prigrow XIV Medium

TM014 500 ml
EUR 195

Prigrow VII Medium

TM017 500ml
EUR 145

Prigrow XIII Medium

TM013 500 ml
EUR 125

Prigrow VIII Medium

TM018 500ml
EUR 145

Prigrow XVIII Medium

TM039 500 ml
EUR 195

EXS III Basal Medium

30630164-1 25 L
EUR 23.69

EXS III Basal Medium

30630164-2 50 L
EUR 43

Artificial Seawater Nutrient Medium (III)

PT153-10X1L 1 unit
EUR 12.95
Description: Artificial Seawater Nutrient Medium (III)

Artificial Seawater Nutrient Medium (III)

PT153-25L 1 unit
EUR 25.14
Description: Artificial Seawater Nutrient Medium (III)

Artificial Seawater Nutrient Medium (III)

PT153-5L 1 unit
EUR 5.63
Description: Artificial Seawater Nutrient Medium (III)

Hosta Rooting Medium Stage III

30630167-3 25 L
EUR 61.84

Hosta Rooting Medium Stage III

30630167-4 50 L
EUR 116.82

EXS III Basal Salt Medium, w/ Adenine

30630163-1 25 L
EUR 21.41

EXS III Basal Salt Medium, w/ Adenine

30630163-2 50 L
EUR 36.33

KATO III [KATO 3] Cells Complete Medium

CM-0372-125mL4 125 mL×4
EUR 108
Description: Complete Growth Medium

Pringsheim's Medium

M698-100G 1 unit
EUR 23.3
Description: Pringsheim's Medium

Cooked M Medium (R.C. Medium)

M149-100G 1 unit
EUR 27.32
Description: Cooked M Medium (R.C. Medium)

Cooked M Medium (R.C. Medium)

M149-500G 1 unit
EUR 130.49
Description: Cooked M Medium (R.C. Medium)

BAT Medium (Alicyclobacillus Medium)

M1561-500G 1 unit
EUR 43.27
Description: BAT Medium (Alicyclobacillus Medium)

Lowenstein - Jensen Medium (L.J. Medium)

MM162-100G 1 unit
EUR 12.1
Description: Lowenstein - Jensen Medium (L.J. Medium)

Lowenstein - Jensen Medium (L.J. Medium)

MM162-500G 1 unit
EUR 34.85
Description: Lowenstein - Jensen Medium (L.J. Medium)

MSC Prime Medium, 25x

TBS8022-01 1 mL
EUR 76

MSC Prime Medium, 25x

TBS8022-05 5 mL
EUR 268

MSC Prime Medium, 25x

TBS8022-25 25 mL
EUR 968

ATCC Medium 1754: PETC Medium (Tetra Pac

M2104-500G 1 unit
EUR 63.72
Description: ATCC Medium 1754: PETC Medium (Tetra Pac

Human iPS Growth Medium (PSGro Medium)

TBS8023 500 mL
EUR 189

Medium 199

C0012-01 RT 500 mL Bottle
EUR 34

Rat Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-25023 5 x 100 ml Ask for price

Phyto Proteose Agar Medium Base 
(KB Medium)

PHM008-100G 1 unit
EUR 13.81
Description: Phyto Proteose Agar Medium Base 
(KB Medium)

Phyto Proteose Agar Medium Base 
(KB Medium)

PHM008-500G 1 unit
EUR 60.56
Description: Phyto Proteose Agar Medium Base 
(KB Medium)

Human Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium

9-46043 5 x 100 ml Ask for price

EC MEDIUM

E05-100-10kg 10 kg
EUR 976.8

EC MEDIUM

E05-100-2Kg 2 Kg
EUR 259.2

EC MEDIUM

E05-100-500g 500 g
EUR 114

DC MEDIUM

D04-117-10kg 10 kg
EUR 1894.8

DC MEDIUM

D04-117-2kg 2kg
EUR 458.4

DC MEDIUM

D04-117-500g 500 g
EUR 168

A Medium

DJ1018 100g
EUR 101.76

M9 Medium

SD7024 250g
EUR 86.1

YM Medium

SD7031 250g
EUR 84.54

VP Medium

M662-500G 1 unit
EUR 52.25
Description: VP Medium

E.T. Medium

M854-500G 1 unit
EUR 36.34
Description: E.T. Medium

E.T. Medium

M854-5KG 1 unit
EUR 355.04
Description: E.T. Medium

HS Medium

M245-500G 1 unit
EUR 34.73
Description: HS Medium

X6 MEDIUM

X8458 10L
EUR 30.71

LB Medium

TBS8056 1L
EUR 36

OF Medium - 500G

MED1664 500G
EUR 89.1

NLN Medium

30630102-2 25 L
EUR 19.28

NLN Medium

30630102-3 50 L
EUR 39.2

TAP Medium

30639002-1 100 g
EUR 48.32

TAP Medium

30639002-2 500 g
EUR 200.66

HLP MEDIUM

H08-107-10kg 10 kg
EUR 2733.6

HLP MEDIUM

H08-107-2Kg 2 Kg
EUR 640.8

HLP MEDIUM

H08-107-500g 500 g
EUR 218.4

SIM MEDIUM

S19-110-10kg 10 kg
EUR 1225.2

SIM MEDIUM

S19-110-2kg 2kg
EUR 313.2

SIM MEDIUM

S19-110-500g 500 g
EUR 128.4

SOB MEDIUM

S19-124-10kg 10 kg
EUR 1158

SOB MEDIUM

S19-124-2kg 2kg
EUR 298.8

SOB MEDIUM

S19-124-500g 500 g
EUR 124.8

M63 Medium

SD7033 250g
EUR 86.1

RWC Medium

M500-100G 1 unit
EUR 15.93
Description: RWC Medium

RWC Medium

M500-500G 1 unit
EUR 43.27
Description: RWC Medium

PNY Medium

M835-100G 1 unit
EUR 34.17
Description: PNY Medium

PNY Medium

M835-500G 1 unit
EUR 103.52
Description: PNY Medium

SIM Medium

M181-100G 1 unit
EUR 12.27
Description: SIM Medium

SIM Medium

M181-500G 1 unit
EUR 35.37
Description: SIM Medium

AKI Medium

M1879-500G 1 unit
EUR 36.24
Description: AKI Medium

MIU Medium

LQ092-25X10ML 1 unit
EUR 14.98
Description: MIU Medium

MIU Medium

LQ092-50X10ML 1 unit
EUR 24.33
Description: MIU Medium

SIM Medium

GM181-500G 1 unit
EUR 40.65
Description: SIM Medium

YEP MEDIUM

Y889 1L
EUR 75.28

MSC Medium

TBS8021 500 mL
EUR 245

SOB Medium

TBS8057 1L
EUR 45

SOC Medium

TBS8058 100 mL
EUR 35

VALI Medium

0450 500 ml
EUR 177.1

BEGM Medium

0451 500 ml
EUR 89.6

TM4G Medium

30630177-2 25 L
EUR 22.1

TM4G Medium

30630177-3 50 L
EUR 41.14

mD5A MEDIUM

M5516 10L
EUR 258.64

M9CA Medium

SD7025 250g
EUR 86.1

Burk's Medium

M707-500G 1 unit
EUR 41.14
Description: Burk's Medium

RM-01 Refeed Medium (zero-Serum Refeed medium)

10-310100 100 mL Ask for price
Description: A defined medium with EMEM with EBSS, and Glutamine. Sterile.

White's Medium

30630124-3 25 L
EUR 31.67

White's Medium

30630124-4 50 L
EUR 43.46

TYGPN Medium

SD7032 250g
EUR 84.01

Thiol Medium

M852-500G 1 unit
EUR 42.8
Description: Thiol Medium

Craig's Medium

M974-500G 1 unit
EUR 41.3
Description: Craig's Medium

Wurtz Medium

M1065-500G 1 unit
EUR 44.15
Description: Wurtz Medium

Kaper's Medium

M1169-500G 1 unit
EUR 51.41
Description: Kaper's Medium

Insect Cell Medium: TNM-FH Insect Culture Medium

ABP-MED-10001 1 liter Ask for price

Heller Medium

30630036-2 25 L
EUR 25.45

Heller Medium

30630036-3 50 L
EUR 43

Jensen's Medium

30630039-3 25 L
EUR 40.58

Jensen's Medium

30630039-4 50 L
EUR 77.28

Litvay Medium

30630048-3 25 L
EUR 29.72

Litvay Medium

30630048-4 50 L
EUR 40.38

Nitsch Medium

30630095-2 25 L
EUR 25.16

Nitsch Medium

30630095-3 50 L
EUR 43.37

Heller's Medium

CP014-010 10X1L
EUR 118.8

Heller's Medium

CP014-500 50L
EUR 151.2

Jensen's Medium

M710-100G 1 unit
EUR 15.93
Description: Jensen's Medium

Jensen's Medium

M710-500G 1 unit
EUR 41.04
Description: Jensen's Medium

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