nanovezels bevorderde de bijlage en vooruitgang van de menselijke endotheliale cellen in statische en dynamische

Tweelingresultaten van niet-coderende RNA’s (ncRNA’s) in de meeste vormen van kanker Stamcelbiologie

De identificatie van de meeste kankerstamcellen (CSC’s) als initiatoren van carcinogenese heeft een revolutie teweeggebracht in de periode van de meeste kankeranalyses en ons begrip van de keuzes voor het verhelpen van ziekten. Extra CSC-opties, samen met zelfvernieuwing en migrerende en invasieve mogelijkheden, hebben deze cellen extra gerechtvaardigd als vermeende diagnostische, prognostische en therapeutische doelen.

Gezien de CSC-plasticiteit is de identificatie van CSC-gerelateerde biomarkers een zware last geweest bij CSC- karakterisering en therapeutische concentratie. In de afgelopen lange tijd heeft een overtuigende hoeveelheid bewijs cruciale regelgevende mogelijkheden van niet-coderende RNA’s (ncRNA’s) op de unieke opties van CSC’s aangetoond.

We weten nu dat ncRNA’s kunnen binnendringen in signaalroutes, erg belangrijk voor het onderhoud van het CSC-fenotype, vergelijkbaar met Notch, Wnt en Hedgehog. Hier hebben we het over de veelzijdige bijdrage van microRNA’s (miRNA’s), lange niet-coderende RNA’s (lncRNA’s) en ronde RNA’s (circRNA’s) , als consultant ncRNA-cursussen, bij het ondersteunen van de CSC-achtige eigenschappen, naast de onderliggende moleculaire mechanismen van hun beweging in gevarieerde CSC-variëteiten. We praten verder over het gebruik van CSC-gerelateerde ncRNA’s als vermeende biomarkers van buitensporige diagnostische, prognostische en therapeutische waarde.

Electrospun polyurethaan / poly (ɛ-caprolacton) nanovezels bevorderde de bijlage en vooruitgang van de menselijke endotheliale cellen in statische en dynamische aangepaste voorwaarden

• Bij deze studie werd de angiogene functionaliteit van menselijke endotheelcellen bestudeerd na 72 uur op de grond van polyurethaan- polycaprolacton (PU/PCL) steigers te zijn uitgeplaat . In deze studie zijn cellen ingedeeld in 5 totaal verschillende groepen, samen met PU, PU/PCL (2:1), PU/PCL (1:1); PU/PCL (1:2); en PCL.
• Info onthulde dat de PU/PCL (2:1) samenstelling de volgende modulus en breekpunt had in tegenstelling tot de andere groepen (p<0,05). In vergelijking met de andere groepen had de PU/PCL-steiger met een molverhouding van twee:1 een lagere contacthoek θ en een hogere trekspanning (p<0,05).
• De indicatieve meting van de PU-nanovezels werd verlaagd na de toevoeging van PCL (p<0,05). Op basis van onze informatie veroorzaakte de gewoonte van endotheelcellen op basis van PU/PCL (2:1) geen nitrosatieve stress en cytotoxische resultaten onder statische omstandigheden in vergelijking met cellen die waren uitgeplaat op een conventionele plastic vloer (p>0,05).
• Op basis van informatie uit de statische stand van zaken, hebben we een buisvormige PU/PCL (2:1) assemblage gefabriceerd voor zes dagen durende dynamische cel op maat gemaakte inside-loop air-lift bioreactoren. Scanning-elektronenmicroscopie bevestigde de hechting van endotheelcellen aan de luminale vloer van de PU/PCL-steiger. Cellen zijn afgeplat en uitgelijnd onder de aangepaste medium beweging. Immunofluorescentiebeeldvorming bevestigde de hechting van cellen aan de luminale vloer, aangegeven door blauwe kernen op de luminale vloer.
• Deze informatie toonde aan dat de uitrusting van PU/PCL-substraat de trein van endotheelcellen zou kunnen stimuleren onder statische en dynamische omstandigheden.

Driedimensionale celculturen als een in vitro systeem voor prostaat Modellering van de meeste vormen van kanker en ontdekking van geneesmiddelen

Gedurende het laatste decennium heeft de knowhow op het gebied van driedimensionale (3D) celgebruik verschillende nieuwsgierigheid opgedaan die toe te schrijven is aan het potentieel om de in vivo groep en micro-omgeving van in vitro gekweekte meeste kankercellen beter te recapituleren.

Het is veelbetekenend dat 3D-tumormodes verschillende totaal verschillende eigenschappen hebben aangetoond in tegenstelling tot standaard tweedimensionale (2D) culturen en een opvallende hyperlink hebben aangebracht tussen de laatste en dierproeven.

Eigenlijk kenmerken 3D-celculturen een nuttig platform voor de identificatie van de natuurlijke keuzes van de meeste kankercellen, samen met voor de screening van nieuwe antitumormakelaars. De huidige overweging is gericht op het samenvatten van de meest voorkomende aangepaste methoden en functies van 3D-cellen , met een focus op het modelleren van de meeste prostaatkankers en het ontdekken van geneesmiddelen.

M9 Medium
SD7024	Bio Basic	250g	EUR 86.1

YM Medium
SD7031	Bio Basic	250g	EUR 84.54

HLP MEDIUM
H08-107-10kg	Alphabiosciences	10 kg	EUR 2733.6

HLP MEDIUM
H08-107-2Kg	Alphabiosciences	2 Kg	EUR 640.8

HLP MEDIUM
H08-107-500g	Alphabiosciences	500 g	EUR 218.4

M63 Medium
SD7033	Bio Basic	250g	EUR 86.1

SIM MEDIUM
S19-110-10kg	Alphabiosciences	10 kg	EUR 1225.2

SIM MEDIUM
S19-110-2kg	Alphabiosciences	2kg	EUR 313.2

SIM MEDIUM
S19-110-500g	Alphabiosciences	500 g	EUR 128.4

SOB MEDIUM
S19-124-10kg	Alphabiosciences	10 kg	EUR 1158

SOB MEDIUM
S19-124-2kg	Alphabiosciences	2kg	EUR 298.8

SOB MEDIUM
S19-124-500g	Alphabiosciences	500 g	EUR 124.8

Ec Medium 500gm
231430	Scientific Laboratory Supplies	EACH	EUR 176.4

TCBS Medium
MED1188	Scientific Laboratory Supplies	PK10	EUR 9.6

M9CA Medium
SD7025	Bio Basic	250g	EUR 86.1

Sob Medium 500gm
244310	Scientific Laboratory Supplies	EACH	EUR 261.6

Rat Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium
9-25023	CHI Scientific	5 x 100 ml	Ask for price

DNase Medium
MED1196	Scientific Laboratory Supplies	PK10	EUR 10.8

TYGPN Medium
SD7032	Bio Basic	250g	EUR 84.01

Heller's Medium
CP014-010	GenDepot	10X1L	EUR 118.8

Heller's Medium
CP014-500	GenDepot	50L	EUR 151.2

Human Cartilage Growth Medium PrimaCell: Normal Articular Cartilage Growth Medium
9-46043	CHI Scientific	5 x 100 ml	Ask for price

Insect Cell Medium: TNM-FH Insect Culture Medium
ABP-MED-10001	Allele Biotech	1 liter	Ask for price

Mounting Medium
4300	Virostat	3 ml	EUR 216.6
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein.

Advanced Medium
C0003-04	Addexbio	RT 500 mL Bottle	EUR 123.6

COLIFORM MEDIUM
C03-127-10kg	Alphabiosciences	10 kg	EUR 1588.8

COLIFORM MEDIUM
C03-127-2kg	Alphabiosciences	2kg	EUR 392.4

COLIFORM MEDIUM
C03-127-500g	Alphabiosciences	500 g	EUR 150

Hoagland's Medium
CP015-010	GenDepot	10X1L	EUR 118.8

Hoagland's Medium
CP015-500	GenDepot	50L	EUR 151.2

Insect Cell Medium: Serum-Free Insect Culture Medium
ABP-MED-10002	Allele Biotech	1 liter	Ask for price

RPMI 1640 Medium
abx098879-500ml	Abbexa	500 ml	EUR 210

A-1 MEDIUM
A01-116-10kg	Alphabiosciences	10 kg	EUR 1240.8

A-1 MEDIUM
A01-116-2Kg	Alphabiosciences	2 Kg	EUR 316.8

A-1 MEDIUM
A01-116-500g	Alphabiosciences	500 g	EUR 129.6

MCDB 131 Medium
CM034-050	GenDepot	500ml	EUR 102

MCDB 131 Medium
CM034-300	GenDepot	6x500ml	EUR 198

MCDB 131 Medium
CM034-310	GenDepot	10x500ml	EUR 262.8

MCDB 131 Medium
CM034-320	GenDepot	20x500ml	EUR 408

MCDB 131 Medium
CM034-350	GenDepot	50x500ml	EUR 702

Medium 199/BCDB 105
C0012-02	Addexbio	RT 500 mL Bottle	EUR 181.2

Insect Cell Medium: Grace?s Insect Medium (Unsupplemented)
ABP-MED-10004	Allele Biotech	1 liter	Ask for price

Isp Medium 2 500gm
277010	Scientific Laboratory Supplies	EACH	EUR 356.4

Isp Medium 4 500gm
277210	Scientific Laboratory Supplies	EACH	EUR 340.8

BPS Medium 1
79259	BPS Bioscience	100 ml	EUR 120
Description: This liquid BPS Medium is optimized for use with many BPS Reporter Kits including_x000D_ • 60612: AP1 Reporter Kit (JNK Pathway)_x000D_ • 60514: ARE Reporter Kit (Nrf2 Antioxidant Pathway)_x000D_ • 60611: CRE/CREB Reporter Kit (cAMP/PKA Signaling Pathway)_x000D_ • 60614: NF-κB Reporter Kit (NF-κB Signaling Pathway)_x000D_ • 60509: Notch1/CSL Reporter Kit (Notch Signaling Pathway)_x000D_ • 60654: SBE Reporter Kit (TGFβ/SMAD signaling pathway)_x000D_ • 60511: SRE Reporter Kit (MAPK/ERK Signaling Pathway)_x000D_ • 60500: TCF/LEF Reporter Kit (Wnt Signaling Pathway)

BPS Medium 7
79260	BPS Bioscience	100 ml	EUR 120
Description: This liquid BPS Medium is optimized for use with select BPS reporter kits.

BPS Medium 8
79385	BPS Bioscience	100 ml	EUR 120
Description: This liquid BPS Medium is optimized for use with the CSF1R/SRE Reporter Kit (BPS Bioscience, #79379).

Thaw Medium 8
79652-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines including NF- κB Reporter (Luc) – THP-1 Cell Line, #79645

Thaw Medium 8
79652-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines including NF- κB Reporter (Luc) – THP-1 Cell Line, #79645

Thaw Medium 9
79665-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including_x000D_• CRE/CREB Reporter Lentivirus #79580 _x000D_• NF-κB Reporter Luciferase Reporter Lentivirus #79564

Thaw Medium 9
79665-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, using • CRE/CREB Reporter Lentivirus #79580 _x000D_• NF-κB Reporter Luciferase Reporter Lentivirus #79564

Thaw Medium 10
79704-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines.

Thaw Medium 10
79704-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines.

Thaw Medium 11
79976-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_NF- κB Reporter (Luc) - Raw 264.7 Cell line (BPS Bioscience 79978)

Thaw Medium 11
79976-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid medium is optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_NF- κB Reporter (Luc) - Raw 264.7 Cell line (BPS Bioscience 79978)

Thaw Medium 12
78074-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including: • Cas9-expressing MDA-MB-231 cells (BPS Bioscience #78069)

Thaw Medium 12
78074-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including: • Cas9-expressing MDA-MB-231 cells (BPS Bioscience #78069)

Thaw Medium 14
78397-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 14
78397-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 15
78412-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 15
78412-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines.

Thaw Medium 16
78647-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for use with tetracycline-inducible system.

Thaw Medium 16
78647-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for use with tetracycline-inducible system.

Thaw Medium 4
60181-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607

Thaw Medium 4
60181-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• CFTR - HEK 293 Recombinant Cell Line #60506• Nav1.8/β2 - HEK293 Recombinant Cell Line #60521• NaV1.7-HEK293 Recombinant Cell Line #60607

Thaw Medium 5
60182-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409

Thaw Medium 5
60182-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Gli Reporter - NIH3T3 Recombinant Cell line (Hedgehog Pathway) #60409

Thaw Medium 6
60183-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604

Thaw Medium 6
60183-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520• NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623 • RARα Reporter - HEK293 Recombinant Cell Line #60503 • RARβ Reporter - HEK293 Recombinant Cell Line #60603 • RARγ Reporter - HEK293 Reporter Cell Line #60604

Thaw Medium 2
60184-1	BPS Bioscience	100 ml	EUR 150
Description: This liquid Cell Thawing Medium is optimized for thawing and plating BPS Bioscience's Jurkat-based cell lines, including• PD-1/NFAT Reporter-Jurkat cell #60535• NFAT Reporter (Luc) - Jurkat Cell Line #60621• NF-κB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540• ADCC Bioassay Effector Cell V variant (High Affinity) #60541Note: once cells are well-established, switch to the appropriate cell growth medium.

Thaw Medium 2
60184-2	BPS Bioscience	500 ml	EUR 675
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including_x000D__x000D_• PD-1/NFAT Reporter-Jurkat cell #60535_x000D__x000D_• NFAT Reporter (Luc) - Jurkat Cell Line #60621_x000D__x000D_• NF-ĸB-Luciferase Reporter (Luc) - Jurkat Cell Line #60651_x000D__x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) #60540_x000D__x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) #60541_x000D__x000D_Note: once cells are well-established, switch to the appropriate cell growth medium.

Thaw Medium 7
60185-1	BPS Bioscience	100 ml	EUR 140
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience cell lines, including• Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520 • NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623

Thaw Medium 7
60185-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Cell Thawing Medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including • Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway) #60520 • NF-κB Reporter (Luc) - HCT116 Recombinant Cell Line #60623

Thaw Medium 3
60186-1	BPS Bioscience	100 ml	EUR 150
Description: This liquid Cell Thaw Medium is optimized for thawing and plating BPS Bioscience's CHO cell-based recombinant cell lines, including• TCR Activator - CHO Recombinant Cell line #60539 • TCR activator / PD-L1 - CHO Recombinant Cell line #60536 It is designed for use in a 37°C incubator with an atmosphere of 5% CO2/95% air. Note: once cells are well established, switch to an appropriate cell growth medium.

Thaw Medium 3
60186-2	BPS Bioscience	500 ml	EUR 645
Description: This liquid thaw medium is optimized for thawing and plating select BPS Bioscience recombinant cell lines, including_x000D__x000D_• TCR Activator - CHO Recombinant Cell line #60539 _x000D_• TCR activator / PD-L1 - CHO Recombinant Cell line #60536 _x000D__x000D_• CD27 CHO-K1 Stable Recombiant Cell Line #60624 _x000D__x000D_• PD-L1 - CHO Recombinant Cell Line #60543 _x000D__x000D_• NF-κB Reporter (Luc) - CHO-K1 Recombinant Cell Line #60622_x000D_• CD137L (4-1BBL) CHO-K1 Recombinant Cell Line #60523_x000D__x000D_It is designed for use in a 37°C incubator with an atmosphere of 5% CO2/95% air. _x000D_Note: once cells are well established, switch to an appropriate cell growth medium._x000D_

Thaw Medium 1
60187-1	BPS Bioscience	100 ml	EUR 155
Description: This liquid HEK293 Cell Thawing Medium is optimized for thawing and plating BPS Bioscience's HEK293 recombinant cell lines.

Thaw Medium 1
60187-2	BPS Bioscience	500 ml	EUR 695
Description: This liquid Thaw Medium is optimized for thawing and plating BPS Bioscience's HEK293 recombinant cell lines.

10mL S.O.C. Medium
46-003-CR	Scientific Laboratory Supplies	PK10	EUR 122.4

Of Basal Medium 500gm
268820	Scientific Laboratory Supplies	EACH	EUR 224.4

Medium 199 (Mod) Powder
90-050PB	Scientific Laboratory Supplies	EACH	EUR 93.48

Assay Medium 1B
79617-1	BPS Bioscience	100 ml	EUR 135
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TGF/SMAD Signaling Pathway SBE Reporter - HEK293 Cell Line #60653 _x000D_• AP-1 Reporter - HEK293 Recombinant Cell Line (JNK signaling pathway) #60405

Assay Medium 1B
79617-2	BPS Bioscience	500 ml	EUR 675
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TGF/SMAD Signaling Pathway SBE Reporter - HEK293 Cell Line #60653 _x000D_• AP-1 Reporter - HEK293 Recombinant Cell Line (JNK signaling pathway) #60405

Assay Medium 2B
79619-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line -#60628

Assay Medium 2B
79619-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line -#60628

Assay Medium 5A
79620-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience cell lines.

Assay Medium 5A
79620-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience cell lines.

Assay Medium 2A
79621-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) - Jurkat Recombinant Cell Line #60540 _x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) - Jurkat Recombinant Cell Line #60541

Assay Medium 2A
79621-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• ADCC Bioassay Effector Cell F variant (Low Affinity) - Jurkat Recombinant Cell Line #60540 _x000D_• ADCC Bioassay Effector Cell V variant (High Affinity) - Jurkat Recombinant Cell Line #60541

Assay Medium 2C
79623-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TIGIT / NFAT Reporter - Jurkat Recombinant Cell Line - #60538_x000D_• CD155/TCR Activator - CHO Recombinant Cell Line -#60548

Assay Medium 2C
79623-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including:_x000D_• TIGIT / NFAT Reporter - Jurkat Recombinant Cell Line, #60538 • CD155/TCR Activator - CHO Recombinant Cell Line, #60548

Assay Medium 7B
79718-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience cell lines._x000D_Optimized for use with Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway), #60520

Assay Medium 7B
79718-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid assay medium is optimized for use in cellular assays in select BPS Bioscience cell lines._x000D_Optimized for use with Myc Reporter (Luc) - HCT116 Recombinant Cell Line (Myc Signaling Pathway), #60520

Assay Medium 1A
79805-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including TrkA / SRE Reporter - HEK293 Recombinant Cell Line (#79798)

Assay Medium 1A
79805-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Assay Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including TrkA / SRE Reporter - HEK293 Recombinant Cell Line (#79798)

Assay Medium 2C
78544-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Assay Medium 2C
78544-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Assay Medium 7A
78673-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay

Assay Medium 7A
78673-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay

Assay Medium 1C
78674-1	BPS Bioscience	100 ml	EUR 120
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay.

Assay Medium 1C
78674-2	BPS Bioscience	500 ml	EUR 600
Description: This liquid Assay Medium is optimized for use with BPS Bioscience cell-based assay.

H Medium Broth
DJ1019	Bio Basic	100g	EUR 73.06

OF BASAL MEDIUM
O15-100-10kg	Alphabiosciences	10 kg	Ask for price

OF BASAL MEDIUM
O15-100-2kg	Alphabiosciences	2kg	Ask for price

OF BASAL MEDIUM
O15-100-500g	Alphabiosciences	500 g	Ask for price

NeuroProgenitor Medium
NM42400	Neuromics	125 ml	EUR 364.8

Growth Medium 1A
79528	BPS Bioscience	500 ml	EUR 715
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 3B
79529	BPS Bioscience	500 ml	EUR 715
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 2B
79530	BPS Bioscience	500 ml	EUR 700
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol._x000D_Optimized for use with:_x000D_IL-2 Jurkat Luciferase Reporter - Jurkat Cell Line #60481 NF-kB Luciferase Reporter - Jurkat Cell Line #60651 Foxp3 Reporter (Luc) - Jurkat Recombinant Cell Line #60628

Growth Medium 1B
79531	BPS Bioscience	500 ml	EUR 755
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 1C
79532	BPS Bioscience	500 ml	EUR 755
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 1K
79533	BPS Bioscience	500 ml	EUR 715
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 5A
79534	BPS Bioscience	500 ml	EUR 715
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 4A
79535	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 1D
79536	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 3C
79537	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 1E
79538	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 3D
79539	BPS Bioscience	500 ml	EUR 735
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 1F
79540	BPS Bioscience	500 ml	EUR 755
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 5B
79541	BPS Bioscience	500 ml	EUR 685
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 6A
79542	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 7A
79543	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 1G
79544	BPS Bioscience	500 ml	EUR 755
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Optimized for use with GR-GAL4 Reporter (Luc) -HEK293 Recombinant Cell Line (Glucocorticoid Receptor Pathway), #60655, and CD137/NF-κB Reporter - HEK293 Recombinant Cell Line, #79289

Growth Medium 7B
79545	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines._x000D_Optimized for use with Myc Reporter (Luc) - HCT116 Recombinant Cell Line, #60520.

Growth Medium 1H
79546	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.Optimized for Use With: T-Rex HEK293 Recombinant Cell Line (BPS Bioscience, #71227)

Growth Medium 1J
79552	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 3E
79553	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 4B
79554	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including: • Nav1.8/β2 - HEK293 Recombinant Cell Line #60521

Growth Medium 1L
79555	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 2C
79592	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol._x000D_Optimized for use with:_x000D_ GITR/NF-κB Luciferase Reporter (Luc) - Jurkat Cell Line #60546_x000D_

Growth Medium 4C
79631	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including: • CFTR - HEK 293 Recombinant Cell Line - #60506

Growth Medium 2E
79638	BPS Bioscience	500 ml	EUR 715
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol._x000D_Optimized for use with:_x000D_CRE/CREB Reporter (Luc) - Jurkat Cell Line - #79636_x000D_

Growth Medium 2D
79639	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.Optimized for Use With: TCR Activator - Raji Cell Line (BPS Bioscience, #60556)

Growth Medium 8A
79653	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol._x000D_Optimized for Use With: _x000D_NF- κB Reporter (Luc) - THP-1 Cell Line - #79645

Growth Medium 6B
79657	BPS Bioscience	500 ml	EUR 700
Description: This liquid Growth Medium is optimized for use in cellular assays in select BPS Bioscience's cell lines, including: • NF-kB Reporter (Luc) - A549 Stable Cell Line #60625

Growth Medium 2F
79669	BPS Bioscience	500 ml	EUR 685
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol._x000D_ Optimized for use with ICOS NFAT - Jurkat Cell Line, #79668

Growth Medium 1M
79723	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol._x000D_ Optimized for Use With: CSF1R / SRE - Reporter HEK293 Recombinant Cell Line (#79380)

Growth Medium 2G
79734	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines._x000D_Optimized for use with: ADCC Bioassay Effector Cell - Mouse - Jurkat Recombinant Cell Line, (BPS #79733)

Growth Medium 2H
79784	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines._x000D_ Optimized for use with: Anti-BCMA CAR Jurkat/NFAT (Luciferase) Reporter Cell Line (BPS #79694)

Growth Medium 1N
79801	BPS Bioscience	500 ml	EUR 755
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Follow the culturing instructions on the designated protocol.

Growth Medium 2J
79812	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines._x000D_ Optimized for use with: LAG3 / IL-2 Reporter - Jurkat Recombinant Cell Line (BPS# 79813)

Growth Medium 3F
79829	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With: BCMA / GLuc - CHO Recombinant Cell Line #79830

Growth Medium 3G
79882	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines and lentivirus._x000D_Optimized for Use with_x000D_ FcGRIIIA (CD16a) Lentivirus, FcER1G Lentivirus

Growth Medium 3J
79974	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines._x000D_Optimized for use with: ACE2 - CHO Recombinant Cell Line (BPS Bioscience #79959)

Growth Medium 3K
78041	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. _x000D_Optimized for Use With_x000D_ CD19 / BCMA / Firefly Luciferase - CHO Recombinant Cell Line (BPS Bioscience, #78030)

Growth Medium 7C
78076	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell pools_x000D_Optimized for use With: Cas9-expressing HCT116 cell pool (BPS Bioscience, #78073)

Growth Medium 6C
78077	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell pools._x000D_Optimized for use with: Cas9-expressing A549 cell pool (BPS Bioscience, #78072)

Growth Medium 2K
78078	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell pools._x000D_Optimized for use with: Cas9-expressing Jurkat cell pool (BPS Bioscience, #78070) Cas9-expressing Raji cell pool (BPS Bioscience, #78071)

Growth Medium 2L
78094	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell pools._x000D_Optimized for Use With: CRISPRa (SAM) Jurkat Cell Pool (BPS Bioscience, #78080)

Growth Medium 1P
78095	BPS Bioscience	500 ml	EUR 685
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_ TMPRSS2 - Vero E6 Recombinant Cell Line (BPS Bioscience, #78081)

Growth Medium 1Q
78096	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell pools_x000D_Optimized for Use With:_x000D_ Cas9-Expressing Neuro2A Cell Pool (BPS Bioscience, #78087)

Growth Medium 3L
78104	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 1R
78180	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell pools.Optimized for Use With: Cas9-Expressing MCF7 Cell Pool (BPS Bioscience #78179)

Growth Medium 2M
78181	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 2N
78196	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 7D
78200	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines

Growth Medium 1S
78221	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.Optimized for Use With: CRISPRa (SAM) HeLa Cell Line (BPS Bioscience, #78193), CRISPRa (SAM) HepG2 Cell Line (BPS Bioscience, #78194)

Growth Medium 6D
78222	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.Optimized for Use With: CRISPRa (SAM) HEK293 Cell Line (BPS Bioscience, #78192)

Growth Medium 2P
78354	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 3M
78377	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 1T
78379	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 2Q
78380	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 6E
78385	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 2R
78411	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 2S
78430	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 3N
78534	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 6F
78540	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 1U
78548	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines

Growth Medium 1V
78551	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 3A
60188	BPS Bioscience	500 ml	EUR 755
Description: This liquid Growth Medium is a complete medium optimized to culture select CHO and CHO-K1 cell lines.

Growth Medium 2A
60190	BPS Bioscience	500 ml	EUR 755
Description: This liquid Growth Medium is a complete medium optimized to culture select Jurkat cell lines.

Fluid Thio Medium 500gm
225650	Scientific Laboratory Supplies	EACH	EUR 139.2

Difco 2xYT Medium
244020	Scientific Laboratory Supplies	EACH	EUR 132

Growth Medium 10A
79835	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines. Optimized for use with Firefly Luciferase -RPMI 8226 Recombinant Cell Line - #79834

Growth Medium 11A
79977	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines._x000D_Optimized for Use With:_x000D_NF- κB Reporter (Luc) - Raw 264.7 Cell line (BPS Bioscience #79978)

Growth Medium 12A
78075	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell pools_x000D_Optimized for use With: Cas9-expressing MDA-MB-231 cell pool (BPS Bioscience, #78069)

Growth Medium 10B
78381	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 14A
78398	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

Growth Medium 15A
78413	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines

Growth Medium 14B
78416	BPS Bioscience	500 ml	EUR 650
Description: This liquid Growth Medium is a complete medium optimized to culture select BPS Bioscience cell lines.

CARY & BLAIR MEDIUM
C03-131-10kg	Alphabiosciences	10 kg	EUR 2622

CARY & BLAIR MEDIUM
C03-131-2Kg	Alphabiosciences	2 Kg	EUR 616.8

CARY & BLAIR MEDIUM
C03-131-500g	Alphabiosciences	500 g	EUR 211.2

Williams' Medium E
CM063-050	GenDepot	500ml	EUR 109.2

Williams' Medium E
CM063-300	GenDepot	6x500ml	EUR 319.2

Williams' Medium E
CM063-310	GenDepot	10x500ml	EUR 375.6

Williams' Medium E
CM063-320	GenDepot	20x500ml	EUR 724.8

Williams' Medium E
CM063-350	GenDepot	50x500ml	EUR 1170

EC MEDIUM, MODIFIED
E05-108-10kg	Alphabiosciences	10 kg	EUR 1029.6

EC MEDIUM, MODIFIED
E05-108-2Kg	Alphabiosciences	2 Kg	EUR 271.2

EC MEDIUM, MODIFIED
E05-108-500g	Alphabiosciences	500 g	EUR 117.6

Temperatuurgevoelige methylcellulose-hyaluronhydrogel als een 3D-celcultuurmatrix

• Deze studie onderzocht de uitrusting van een temperatuurgevoelige methylcellulose-hyaluronzuur (MC-HA) hydrogel om de voortgang van 3D-cellen in vitro te helpen. Het voorbereidende werk concentreerde zich op de voorbereiding van hydrogels voor 3D-gebruik , aangenomen door onderzoeken naar de natuurlijke compatibiliteit van hydrogel-elementen en optimalisatie van de aangepaste celomgeving.

• Evaluatie van de levensvatbaarheid en proliferatie van HCT116-cellen die in de MC-HA-hydrogel waren gekweekt, werd gebruikt om de combo-samenstelling te controleren om een hydrogel te ontwerpen met optimale eigenschappen om de celvooruitgang te helpen.

• Twee zeer belangrijke factoren met betrekking tot de bruikbaarheid van de voorgestelde polymere matrix in 3D-celgebruik zijn aangetoond: i) 3D-gekweekte celaggregaten zullen waarschijnlijk uit de matrix worden gelanceerd/teruggewonnen door middel van een fragiel verloop dat de levensvatbaarheid van de cellen kan verdedigen, en ii) de hydrogelmatrix kan worden gebruikt in plaatformaat met 96 putjes als een dagelijkse techniek die wordt gebruikt bij in vitro weefselspecifieke onderzoeken.

• Het werk vanwege deze realiteit onthult dat MC-HA-hydrogels potentieel bieden voor in vitro 3D-celgebruik als goedkope en goed gedefinieerde alternatieve keuzes voor van dieren afgeleide of geavanceerde synthetische strategieën.

Primordiale kiemcellen van muizen: in vitro aangepaste en conversie naar pluripotente stamcelsporen

Primordiale kiemcellen (PGC’s) zijn de embryonale voorlopers van de gameten. Ongeacht een zeer lange studietijd, in vitro gebruik van PGC’s blijft een belangrijk probleem en heeft vooraf vertrouwd op ongedefinieerde elementen die verwant zijn aan serum en feeders.

Met name PGC’s die voor langere intervallen zijn gekweekt, behouden hun afstammings- ID niet, maar dragen in plaats daarvan conversie naar variëteit pluripotente stamcelstammen die vaak embryonale kiemcellen (EG) worden genoemd als reactie op LIF / STAT3-signalering. Juist hier rapporteren we alle gevestigde en nieuwe methoden om EG-cellen af ​​te leiden, in heel veel verschillende omstandigheden.

We zijn van mening dat grote bezorgdheid over de voortgang van fibroblasten eenvoudigweg niet vereist is voor EG-celconversie. We houden rekening met de stappen die in ons laboratorium zijn genomen om systematisch geavanceerde elementen weg te nemen en een volledig geschetst protocol te regelen dat een aangename conversie van geïsoleerde PGC’s mogelijk maakt naar pluripotente EG-cellen mogelijk maakt.

Daarnaast presenteren we dat PGC’s op maat kunnen hechten en zich vermenigvuldigen zonder de hulp van voedingscellen of serum. Het zal met succes nieuwe benaderingen adviseren om op korte termijn gebruik van PGC’s in geschetste omstandigheden tot stand te brengen.